Dextran CLIO Magnetic Nanoparticles - Fluorescent Dye

General Information
Fluorescent dye functionalized Dextran coated crosslinked iron oxide nanoparticles (CLIO-DYE) have 1-3 superparamagnetic Fe3O4 iron oxide cores (8-10 nm in diameter) imbedded within the matrix of dextran sugar strands of 40 kDa or 70 kDa size. Dextran strands are crosslinked to prevent their disassociation, ensuring long-term biological stability. Fluorophores are covalently conjugated to the nanoparticle surface.
 
Features
Highly magnetic
Highly biocompatible, non-toxic
Fluorescently labeled (AFDye 488, AFDye 555, AFDye 647, or IR750 dye)
Long term stable under physiological conditions
40 kDa and 70 kDa dextran coats are available
Dual-modality magnetic and fluorescence probes
 
Applications
Dual-modality probes for MRI and Fluorescence imaging
In vitroand in vivolabels for cells and tissues
Dual magnetic and florescence cell separation
 
Specifications
Iron Oxide Core Diameter: 8 – 10 nm
Hydrodynamic Diameter: 60 – 80 nm
Polydispersity Index (PDI): < 0.25
Nanoparticle Charge: Between -3 and -0.3 mV
Magnetization:  5 – 30 emu/g (room temperature)
Fluorescent dye: AF488, Cy3, Cy5, or IR750
Shelf life: > 1 year (4°C storage)
Supplied as liquid suspension in water
 
Citations:
Gu, Huan, et al. "Magnetically driven active topography for long-term biofilm control." Nature communications 11.1 (2020): 1-11.
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation. Ashish Tiwari, Narmeen Haj, Betsalel Elgrably, Maria Berihu, Viktor Laskov, Sivan Barash, Shachar Zigron, Hagit Sason, Yosi Shamay, Shiri Karni-Ashkenazi, Maya Holdengreber, Galit Saar, and Katrien Vandoorne. ACS Nano Article ASAP. DOI: 10.1021/acsnano.3c11201
Betsalel Y. Elgrably, Maya Rom, Tehila Alter, Nedaa Kher, Narmeen Haj, Dareen Saab, Ashish Tiwari, Noa Levi, Galit Saar, Shiri Karni-Ashkenazi, Katrien Vandoorne, Nanoparticle immunoimaging reveals metabolic dysfunction fueling CCR2-dependent inflammation in infarcted hearts, Journal of Controlled Release, 2025, 114399, ISSN 0168-3659, https://doi.org/10.1016/j.jconrel.2025.114399