G25 Desalting Gravity Columns

The G25 desalting column utilizes gel filtration chromatography based on cross-linked dextran. This matrix is a porous polymeric substance with a three-dimensional network structure, functioning as a molecular sieve that separates molecules based on size differences. During chromatography, biomolecules larger than the gel pores (such as proteins, peptides, nucleic acids, etc., typically with molecular weights > 5,000 Da) are excluded from the interior of the gel particles. They travel through the spaces between the gel particles and move the fastest, eluting first. In contrast, small molecules (such as buffer components, salts, dyes, etc.) can enter the pores of the gel particles and are eluted last.