PuroSPIN™ Gel Extraction Kit is designed to provide simple and easy-to-use method for extraction and purification of DNA samples (100 bp − 10 kbp) from agarose and PAGE gels. The purification technology is based on dissolving the gel embedded DNA in a buffer solution, followed by nucleic acid binding to silica spin-columns, which allows efficient removal of gel residues and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as PCR, restriction digestion, ligation, labeling, or sequencing.
PuroSPIN™ columns can bind up to 200 μg of DNA product.
PuroSPIN™ Gel Extraction Kit #NK107-50, #NK107-250
Recovered DNA Size 70 bp – 10 kb DNA Recovery 70 – 80 % Column Binding Capacity 200 µg Column Reservoir Capacity 800 µL
Table 1: Extraction of 1500 bp dsDNA strand from 1% agarose gel
PuroSPIN Gel Extraction Kit
Comp. Q Gel Extraction Kit
Comp. B Gel Extraction Kit
Recovery (%) 73 (±7) 71 (±3) 76 (±14) 260/280 1.8 (±0.1) 1.8 (±0.1)
* Data based on 3 head-to-head replicates.