RNase Inhibitor - 10KU (250 μL)

The RNase Inhibitor (Ribonuclease Inhibitor) is a porcine-derived protein that inhibits RNases A, B, and C through noncompetitive binding at a 1:1 molar ratio. Its porcine origin closely resembles the human homolog, ensuring broad compatibility in molecular biology applications.

The inhibitor is active across a pH range of 5–8, with maximal effectiveness observed between pH 7 and 8. To preserve its activity, the storage solution must contain at least 1 mM DTT.
Note: This product does not inhibit RNase 1, RNase T1, S1 Nuclease, RNase H, or RNases derived from Aspergillus species.
 
Source: An E. coli strain that carries the Ribonuclease Inhibitor gene from porcine. 
Purity: ≥95% by SDS-PAGE
Concentration: 40 U/μL
Definition of Activity Unit: One unit is defined as the amount of RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. 
Storage (Dilution) Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM DTT, 50% (v/v) glycerol, pH 7.5. 
 
Inactivation or inhibition: 
Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RNase Inhibitor and release the RNase bound. 
Inactivated by heating at 75°C for 10 min.
 
Storage
Store at -20 °C, valid for 12 months.