Recombinant Bacillus anthracis Thymidylate kinase (tmk)

Shipping Cost Note
Each Recombinant Protein price includes shipping cost to Canada and import fees. Please ask for a discounted quote if ordering multiple Cusabio products.
 
Delivery Time Note
This product is usually imported from the manufacturer after order placement. Please expect longer delivery times of 7-14 days.
 
Catalog No.
CSB-EP007226BQG
 
Product Name
Recombinant Bacillus anthracis Thymidylate kinase (tmk)
 
In Stock
Yes
 
Activity
Not Tested
 
Research Area
Others
 
Uniprot ID
C3LJ02
 
Gene Names
tmk
 
Alternative Names
tmk; BAMEG_0039Thymidylate kinase; EC 2.7.4.9; dTMP kinase
 
Organism
Bacillus anthracis (strain CDC 684 / NRRL 3495)
 
Source
E.coli
 
Expressed Region
1-208aa
 
Protein Length
Full Length
 
Tag Info
N-terminal 6xHis-tagged
 
Target Protein Sequence
MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL
 
MW
27.8 kDa
 
Purity
Greater than 90% as determined by SDS-PAGE.
 
Endotoxin
Not Tested.
 
Form
Liquid or Lyophilized powder
 
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
 
Resonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
 
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. 
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
 
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
 
Relevances
Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.
 
References
"Genome sequence of Bacillus anthracis str. CDC 684."Dodson R.J., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D.Submitted (OCT-2008)
 
Function
Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.