Recombinant Hepatitis B virus genotype D subtype ayw Large envelope protein (S)

Shipping Cost Note
Each Recombinant Protein price includes shipping cost to Canada and import fees. Please ask for a discounted quote if ordering multiple Cusabio products.
 
Delivery Time Note
This product is usually imported from the manufacturer after order placement. Please expect longer delivery times of 7-14 days.
 
Catalog No.
CSB-CF724165HEN
 
Product Name
Recombinant Hepatitis B virus genotype D subtype ayw Large envelope protein (S)
 
In Stock
Yes
 
Activity
Not Tested
 
Research Area
Others
 
Uniprot ID
Q67875
 
Gene Names
S
 
Alternative Names
L glycoprotein;L-HBsAg;LHB;Large S protein;Large surface protein;Major surface antigen
 
Organism
Hepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992) (HBV-D)
 
Source
in vitro E.coli expression system
 
Expressed Region
2-389aa
 
Protein Length
Full Length of Mature Protein
 
Tag Info
N-terminal 6xHis-tagged
 
Target Protein Sequence
GQNLSTSNPLGFFPDHQLDPASRANTANPDWDFNPNKDTWPDANKDGAGAFGLGLTPPHGGLLGWSPQAQGILHTVPANPPPASTNRQTGRQPTPLSPPLRDTHPQAVQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVPTTASPLSSIFSRIGDPVTNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFRGGTTVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRGFIIFLFILLLCLIFLLVLLEYQGMLHVCPLIPGTTTTSTGPCKTCTTPAQGNSMFPSCCCTKTSDGNCTCIPIPSSWAFAKYLWEWASVRFSWLSLLVPFVQWFVGLSPTVWLSAIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
 
MW
43.5 kDa
 
Purity
Greater than 85% as determined by SDS-PAGE.
 
Endotoxin
Not Tested.
 
Form
Liquid or Lyophilized powder
 
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
 
Resonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
 
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. 
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
 
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
 
Relevances
The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. ; The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.
 
References
 
Function