Recombinant Human Alkaline phosphatase, placental type (ALPP) (Active)

Shipping Cost Note
Each Recombinant Protein price includes shipping cost to Canada and import fees. Please ask for a discounted quote if ordering multiple Cusabio products.
 
Delivery Time Note
This product is usually imported from the manufacturer after order placement. Please expect longer delivery times of 7-14 days.
 
Catalog No.
CSB-MP001632HU
 
Product Name
Recombinant Human Alkaline phosphatase, placental type (ALPP) (Active)
 
In Stock
Yes
 
Activity
Yes
 
Research Area
Cancer
 
Uniprot ID
P05187
 
Gene Names
ALPP
 
Alternative Names
Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1
 
Organism
Homo sapiens (Human)
 
Source
Mammalian cell
 
Expressed Region
23-506aa
 
Protein Length
Full Length of Mature Protein
 
Tag Info
C-terminal 10xHis-tagged
 
Target Protein Sequence
IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD
 
MW
54.1 kDa
 
Purity
Greater than 95% as determined by SDS-PAGE.
 
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
 
Form
Lyophilized powder
 
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
 
Resonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
 
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. 
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
 
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
 
Relevances
Alkaline phosphatase that can hydrolyze various phosphate compounds.
 
References
Functional significance of calcium binding to tissue-nonspecific alkaline phosphatase.
Hoylaerts M.F., Van Kerckhoven S., Kiffer-Moreira T., Sheen C., Narisawa S., Millan J.L.
PLoS ONE 10:e0119874-e0119874 (2015)
 
Function