Recombinant Human Glypican-3 (GPC3), partial (Active)

Shipping Cost Note
Each Recombinant Protein price includes shipping cost to Canada and import fees. Please ask for a discounted quote if ordering multiple Cusabio products.
 
Delivery Time Note
This product is usually imported from the manufacturer after order placement. Please expect longer delivery times of 7-14 days.
 
Catalog No.
CSB-MP009705HUc7
 
Product Name
Recombinant Human Glypican-3 (GPC3), partial (Active)
 
In Stock
Yes
 
Activity
Yes
 
Research Area
Cancer
 
Uniprot ID
P51654
 
Gene Names
GPC3
 
Alternative Names
Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; Glypican-3 alpha subunit; Glypican-3 beta subunit
 
Organism
Homo sapiens (Human)
 
Source
Mammalian cell
 
Expressed Region
25-559aa
 
Protein Length
Partial
 
Tag Info
C-terminal 6xHis-tagged
 
Target Protein Sequence
QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVH
 
MW
62.8 kDa
 
Purity
Greater than 95% as determined by SDS-PAGE.
 
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
 
Form
Lyophilized powder
 
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
 
Resonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
 
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. 
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
 
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
 
Relevances
Cell surface proteoglycan.
Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins.
Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation.
Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands.
Positively regulates the non-canonical Wnt signaling pathway.
Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression.
Inhibits the dipeptidyl peptidase activity of DPP4.
Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4.
Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis.
Required for coronary vascular development.
Plays a role in regulating cell movements during gastrulation.
 
References
Expression of the Glypican-3 protein in hepatoma cells.
Grozdanov P.N., Yovchev M.I., Dabeva M.D.
Submitted to EMBL/GenBank/DDBJ databases (JAN-2006)
 
Function