Recombinant Mouse Claudin-18 (Cldn18)-VLPs (Active)

Shipping Cost Note
Each Recombinant Protein price includes shipping cost to Canada and import fees. Please ask for a discounted quote if ordering multiple Cusabio products.
 
Delivery Time Note
This product is usually imported from the manufacturer after order placement. Please expect longer delivery times of 7-14 days.
 
Catalog No.
CSB-MP005498MO(F3)
 
Product Name
Recombinant Mouse Claudin-18 (Cldn18)-VLPs (Active)
 
In Stock
Yes
 
Activity
Yes
 
Research Area
Cancer
 
Uniprot ID
P56857
 
Gene Names
Cldn18
 
Alternative Names
(Claudin-18)
 
Organism
Mus musculus (Mouse)
 
Source
Mammalian cell
 
Expressed Region
1-264aa of Isoform A2.1
 
Protein Length
Full Length of Isoform A2.1
 
Tag Info
N-terminal 6xHis-tagged (This tag can be tested only under denaturing conditions)
 
Target Protein Sequence
MSVTACQGLGFVVSLIGFAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGVIGILVSIFALKCIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFANMLVTNFWMSTANMYSGMGGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSNFKAVSYHASGQNVAYRPGGFKASTGFGSNTRNKKIYDGGARTEDDEQSHPTKYDYV
 
MW
29.1 kDa
 
Purity
The purity information is not available for VLPs proteins.
 
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
 
Form
Lyophilized powder
 
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
 
Resonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃.
Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
 
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. 
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
 
Notes
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.
The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
 
Relevances
"Claudin 18 is a novel negative regulator of bone resorption and osteoclast differentiation."
Linares G.R., Brommage R., Powell D.R., Xing W., Chen S.T., Alshbool F.Z., Lau K.H., Wergedal J.E., Mohan S.
J Bone Miner Res 27:1553-1565(2012)
 
References
 
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.