SUMO Protease (Ulp1), Recombinant His-Tag, 2000 U

A recombinant His-tagged SUMO protease (Ulp1 fragment from S. cerevisiae) that recognises the folded SUMO tag and cleaves SUMO fusion proteins precisely, leaving the native N-terminus. The His tag lets you remove the protease afterwards on a Ni or Co resin.
Recognises the SUMO structure, not a short sequence, for specific cleavage
Active over a wide temperature range, 2-30 °C
N-terminal His tag for easy removal after cleavage
Supplied at 10 U/µL with 10X buffers and a control protein
Enzyme
Ulp1 fragment (S. cerevisiae), N-terminal His tag
Concentration
10 U/µL
Purity
≥ 95%
Unit definition
Cleaves ≥ 85% of 2 µg control substrate in 1 h at 30 °C, pH 8.0
Contents
2000 U (200 µL) protease; two 10X buffers; control protein
Storage
-20 °C, 12 months
Catalogue number
GLN3411-2000U
For research use only. Not for use in diagnostic or therapeutic procedures.