FastStart DNA Polymerase, 5U/μL

FastStart DNA Polymerase is a mixture of anti Taq enzyme monoclonal antibodies and Taq DNA Polymerase suitable for Hot Start PCR. When using Taq enzyme antibodies for PCR amplification, the binding of Taq enzyme antibodies to Taq enzyme inhibits DNA polymerase activity before high-temperature denaturation, which can effectively inhibit non-specific annealing of primers and non-specific amplification caused by primer dimers under low temperature conditions. Taq enzyme antibodies undergo denaturation in the initial DNA denaturation step of PCR reaction, and polymerase activity is restored, achieving a hot start effect. This product does not require special inactivation of Taq enzyme antibodies and can be used under conventional PCR reaction conditions.
 
FastStart DNA Polymerase has 5'→3' DNA polymerase activity and 5'-3' exonase activity, without 3'-5' exonase activity. The enzyme has an elongation rate of 2kb/min and can amplify fragments up to 5kb in length.The amplified PCR product has an "A" base attached to its 3'end, making it suitable for direct T/A cloning. The blocking rate of polymerase activity reaches over 95% at temperatures of 55℃ and below. Heating at 95℃ for 5 seconds can restore DNA polymerase activity.This product has the characteristics of fast extension speed and high amplification efficiency, and is mainly suitable for experiments such as PCR amplification of DNA fragments and DNA sequence determination.
 
Applications
 
Hot start PCR and RT-PCR, ensuring enhanced specificity, sensitivity, and high yield
Targeted amplification of DNA fragments from a variety of DNA sources, supporting a wide range of downstream applications
Incorporation of modified nucleotides into DNA (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP) for labeling purposes
Minimization of carryover contamination between PCR reactions when used with dUTP and Uracil-DNA Glycosylase (UDG)
Production of PCR master mixes for regulated uses (e.g., in vitro diagnostics, quality control), meeting stringent validation requirements
 
Components
 
Component
FSDP01
500 Units
FSDP02
5000 Units
FSDP03
50000 Units
FastStart DNA Polymerase, 5 U/μL
100 μL
1 mL
10 mL
 
Storage
 
-20°C
 
Activity definition
 
Using activated salmon sperm DNA as template/primer, the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acidic insoluble material was defined as 1 active unit (U) at 74°C for 30 minutes.
 
Quality control
After several column purifications, the purity of SDS-PAGE test is more than 99%; no exogenous nuclease activity is detected; no host residual DNA is detected by PCR method; it can effectively amplify single-copy genes in the human genome
 
SKU: FSDP01, FSDP02, FSDP03