Recombinant Streptococcus pneumoniae Immunoglobulin A1 protease (iga), partial

Shipping Cost Note

Each Recombinant Protein price includes shipping cost to Canada and import fees. Please ask for a discounted quote if ordering multiple Cusabio products.

Delivery Time Note

This product is usually imported from the manufacturer after order placement. Please expect longer delivery times of 7-14 days.

Catalog No.

CSB-EP687514FMW

Product Name

Recombinant Streptococcus pneumoniae Immunoglobulin A1 protease (iga), partial

In Stock

YES

Activity

Not Tested

Research Area

Others

Uniprot ID

Q54875

Gene Names

iga

Alternative Names

(IgA1 protease)(IgA-specific zinc metalloproteinase)

Organism

Streptococcus pneumoniae

Source

E.coli

Expressed Region

313-393aa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Target Protein Sequence

NKPELLYREETIETKIDFQEEIQENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTKK

16.1 kDa

Purity

Greater than 90% as determined by SDS-PAGE.

Endotoxin

Not Tested.

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Resonstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevances

Zinc metalloproteinase which cleaves human immunoglobulin A1 (IgA1) in the hinge region, rendering it less efficient in coating the surface of colonizing or invading pneumococci. May be responsible for pneumococcal infection and is potentially involved in distinct stages of pneumococcal disease.

References

"Streptococcus pneumoniae G5 domains bind different ligands." Paukovich N., Redzic J.S., Chi Y.C., Rahkola J.T., Issaian A., Blue A., Hansen K.C., Janoff E.N., Eisenmesser E.Z. Protein Sci 28:1797-1805(2019)

Function