PuroScript™ RT First Strand cDNA Synthesis Kit (With gDNA Remover)

PuroScript™ RT First Strand cDNA Synthesis Kit (With gDNA Remover) uses a modified mutant reverse transcriptase to efficiently synthesize first-strand cDNA from total RNA or mRNA templates. It contains all reagents required for high-quality cDNA synthesis and offers two types of primers for flexibility: Random Hexamer Primers and Oligo(dT)₁₈ Primers. The resulting single-stranded cDNA can be used directly in PCR or qPCR experiments.
 
The PuroScript RT is a mutant reverse transcriptase derived from M-MLV via in vitro evolutionary screening. It lacks RNase H activity, preventing degradation of RNA in the DNA/RNA hybrid during cDNA synthesis, which ensures both high yield and full-length cDNA. Compared to the wild-type enzyme, PuroScript RT has enhanced thermal stability and cDNA synthesis efficiency. It works efficiently at 42–65 °C, and reverse transcription can be completed in as little as 5 minutes, making it ideal for RNA with complex secondary structures.
 
The kit also includes a gDNA Remover reagent, which rapidly eliminates residual genomic DNA from RNA templates prior to reverse transcription. This improves the reliability of downstream qPCR results and allows primer design without the need to span exon-exon junctions.
 
Storage
Store at -20 °C, valid for 12 months. Avoid repeated freezing and thawing.
 
 
 
SKUs: RS3333-50, RS3333-100