PuroScript™ RT First Strand cDNA Synthesis Kit With RNase Inhibitor/gDNA Remover

The PuroScript™ RT First Strand cDNA Synthesis Kit (With RNase Inhibitor/gDNA Remover) is a first-strand cDNA synthesis kit that uses a modified mutant reverse transcriptase to efficiently synthesize cDNA from total RNA or mRNA templates. It contains all reagents required for high-quality cDNA synthesis and offers two primer types for flexibility: Random Hexamer Primers and Oligo(dT)₁₈ Primers. The resulting single-stranded cDNA can be used directly in PCR or qPCR.
PuroScript RT enzyme
Mutant reverse transcriptase derived from M-MLV via in vitro evolutionary screening
Lacks RNase H activity, preventing degradation of RNA in the DNA/RNA hybrid during cDNA synthesis for high yield and full-length cDNA
Enhanced thermal stability and cDNA synthesis efficiency compared with the wild-type enzyme
Works efficiently at 42–65 °C; reverse transcription can be completed in as little as 5 minutes, ideal for RNA with complex secondary structures
Included reagents
RNase Inhibitor to protect RNA integrity during the reaction
gDNA Remover that rapidly eliminates residual genomic DNA from RNA templates before reverse transcription, improving the reliability of downstream qPCR and allowing primer design without spanning exon-exon junctions
Storage
Store at -20 °C; valid for 12 months. Avoid repeated freezing and thawing.
Available sizes
50 reactions – RS3333-50
100 reactions – RS3333-100
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.