PuroTranscript T7 High Yield Transcription Kit

The PuroTranscript T7 High Yield Transcription Kit is a T7 in vitro transcription kit designed for efficient RNA synthesis. It supports a wide range of applications, including blot and in situ hybridization probe generation, ribonuclease protection assays, post-transcriptional modification studies (e.g. RNA splicing and polyadenylation) and in vitro translation.
The kit uses T7 RNA polymerase, which exhibits high specificity for its cognate promoter and efficiently transcribes DNA templates such as plasmids, PCR fragments or synthetic DNA positioned downstream of the T7 promoter. Under optimal conditions, the system can yield over 100 μg of RNA from just 1 μg of DNA template, making it suitable for synthesizing RNA of various lengths and complexities.
Applications
Blot and in situ hybridization probe generation
Ribonuclease protection assays
Post-transcriptional modification studies (e.g. RNA splicing and polyadenylation)
In vitro translation
Kit components
T7 RNA Transcription Enzyme Mix
5× T7 Transcription Reaction Buffer
25 mM NTP Mix
DNase I
Nuclease-Free Water
Control Template (0.5 μg/μL)
Storage
Store at -20 °C. Valid for 12 months. Avoid repeated freezing and thawing.
SKU: RS31021
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.