PuroMIX FAST Pfus 2X PCR Master Mix

PuroMIX FAST Pfus PCR Master Mix is a ready-to-use 2X Pfu PCR master mix containing modified Pfu DNA polymerase, dNTPs and an optimized reaction buffer. It is designed for routine PCR, colony PCR and amplification of complex or high-GC-content templates.
Simply add DNA template, primers and nuclease-free water to reach a final 1X working concentration. The mix offers strong amplification performance with rapid extension rates of 5–15 s/kb, high fidelity and excellent specificity. PCR products are blunt-ended and suitable for direct cloning into flat-end vectors.
For added convenience, the mix includes loading dye, so PCR products can be applied directly to agarose gel electrophoresis.
Key features
2X premix with modified Pfu DNA polymerase, dNTPs and optimized buffer
Extension rate of 5–15 s/kb
High fidelity and excellent specificity
Blunt-ended products for cloning into flat-end vectors
Contains loading dye for direct gel loading
Storage
Store at -20 °C. Valid for 12 months. Avoid repeated freezing and thawing.
Available sizes
1 mL – RS31305-01
5 x 1 mL – RS31305-05
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.