PuroScript™ One-Step RT-PCR Kit

The PuroScript™ One-Step RT-PCR Kit is a single-tube one-step RT-PCR kit in which reverse transcription and PCR amplification occur in the same reaction using RNA and gene-specific primers. No additional reagents are needed during the process – only the RNA template, target gene primers and water are added – minimizing the risk of contamination.
Kit components
RT Mix – contains PuroScript™ Reverse Transcriptase and an RNase Inhibitor
2× One-Step PCR Master Mix – provides PCR DNA polymerase, reaction buffer, dNTPs, Mg²⁺ and RT-PCR stabilizers
Key features
Single-tube RT-PCR: reverse transcription and PCR in the same reaction
PuroScript™ Reverse Transcriptase is an engineered mutant enzyme developed through in vitro evolution, with low RNase H activity, strong processivity and high thermal stability
The DNA polymerase has proofreading ability and generates blunt-ended products
Formulation optimized to significantly enhance amplification efficiency and specificity
Amplicons up to 8 kb in length under the kit's optimized conditions
Storage
Store at -20 °C, valid for 12 months. Avoid repeated freezing and thawing.
Available sizes
50 reactions – RS3335-50
100 reactions – RS3335-100
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.