PuroScript™ All-in-One First Strand cDNA SuperMix (One-Step gDNA Remover)
The PuroScript™ All-in-One First Strand cDNA Synthesis SuperMix for PCR (One-Step gDNA Remover) is a 5× ready-to-use cDNA synthesis master mix (5× PuroScript All-in-One SuperMix for qPCR) designed for first-strand cDNA synthesis, with reagents for genomic DNA (gDNA) removal. During reverse transcription, the gDNA remover efficiently eliminates residual genomic DNA from the RNA template.
The kit also provides a 5× PuroScript All-in-One No-RT Control SuperMix, which lacks reverse transcriptase, so you can verify that the qPCR template originates solely from cDNA.
What's in the SuperMix
- All components needed for reverse transcription: SweScript RT, RNase inhibitor, Random Primers, Oligo(dT)₁₈ Primers, dNTPs and an optimized buffer system
- PuroScript RT – a mutant reverse transcriptase obtained through in vitro evolutionary screening, offering high thermal stability and strong processivity
- gDNA Remover – a thermosensitive DNase approximately 30 times more active than standard DNase I that targets only double-stranded DNA and is inactivated at elevated temperatures, so it does not interfere with subsequent cDNA synthesis
Storage
Store at -20 °C; valid for 12 months. Avoid repeated freezing and thawing.
Available sizes
- 50 reactions – RS3337-50
- 100 reactions – RS3337-100
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.
