PuroScript™ First Strand cDNA Synthesis SuperMix for PCR

The PuroScript™ First Strand cDNA Synthesis SuperMix for PCR is a 5× ready-to-use premix designed specifically for first-strand cDNA synthesis. To perform the reaction, only the RNA template and nuclease-free water need to be added.
The premix contains all essential components for reverse transcription, including PuroScript™ RT, RNase Inhibitor, Oligo(dT)₁₈ Primer, Random Primer, dNTPs and an optimized buffer system. By adjusting the proportion of Oligo(dT)₁₈ and random primers, and using the mutant PuroScript™ RT – an enzyme with high thermal stability and strong processivity developed through in vitro evolutionary screening – the SuperMix enables synthesis of first-strand cDNA fragments up to 15 kb within a temperature range of 42 °C to 55 °C.
The resulting cDNA can be used directly for downstream PCR amplification, offering a simple workflow while minimizing the risk of contamination.
Key features
5× all-in-one premix – just add RNA template and nuclease-free water
Contains PuroScript™ RT, RNase Inhibitor, Oligo(dT)₁₈ Primer, Random Primer, dNTPs and optimized buffer
First-strand cDNA up to 15 kb
Reaction temperature range of 42 °C to 55 °C
Storage
Store at -20 °C. Valid for 12 months. Avoid repeated freezing and thawing.
Available size
50 reactions – RS3336-50
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.