PuroMIX Direct Tissue PCR Kit

The PuroMIX Direct Tissue PCR Kit is engineered for rapid, reliable direct PCR amplification from animal tissues, eliminating the need for conventional DNA extraction. Compatible sample types include fresh or frozen mammalian viscera, nails, hair, zebrafish, Drosophila and cultured cells.
The kit's proprietary Direct Tissue Lysis Buffers A and B work together to efficiently release genomic DNA from micro-tissues. This streamlined lysis bypasses traditional purification steps, minimizing sample loss and significantly reducing experimental time and complexity.
PuroMIX 2X Direct Tissue PCR Mix
A ready-to-use master mix containing all essential PCR components, including:
Modified DNA polymerase with enhanced resistance to inhibitors and high-speed amplification
Optimized enhancers that boost amplification efficiency and fidelity, enabling robust amplification of targets up to 3 kb
Integrated loading dye for direct loading onto agarose gels, allowing fast visualization without compromising PCR performance
Key features
No DNA extraction process
Little sample required
Saves time
Minimizes errors
Storage
Direct Tissue Lysis Buffer A: store at 4 °C
Direct Tissue Lysis Buffer B and PuroMIX 2X Direct Tissue PCR Mix: store at -20 °C
Valid for 12 months; avoid repeated freezing and thawing
Available sizes
1 mL – RS31310-01
5 x 1 mL – RS31310-05
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.