PuroBLUE 2X Universal qPCR Master Mix

PuroBLUE 2X Universal qPCR Master Mix is a 2X SYBR Green I qPCR master mix: a specialized pre-mix for qPCR reactions using the SYBR Green I chimeric fluorescence method.
The core component is a hot-start Taq DNA Polymerase based on the antibody method of confinement, which effectively inhibits non-specific amplification under low-temperature conditions. It is paired with a reaction buffer optimized for qPCR.
Key features
Highly specific and sensitive qPCR reactions
Good standard curve across a wide quantitative range, for accurate and reproducible quantification of the target gene
Contains a special ROX Passive Reference Dye suitable for all qPCR instruments – no need to adjust the ROX concentration on different instruments
Visual colour tracing of reaction setup
Colour tracer dyes
The Master Mix solution includes a blue tracer dye, and a second yellow tracer dye is provided as a 40X concentrate for template dilution. When the template, diluted with the provided yellow dye, is mixed with the blue Master Mix, the colour of the mixture changes to green. The specific colour of each reaction component and of the combined mixture allows visual tracing of the reaction setup steps, minimizing the chance of incorrect solution additions. The spectra of the blue and yellow tracer dyes do not overlap with that of SYBR Green I and therefore do not affect qPCR results.
This product contains 40X yellow template Dilution Buffer.
Storage
Store at -20 °C, valid for 12 months. Avoid freeze-thaw cycles.
Available sizes
1 mL (100 rxns) – RS31326-01
5 x 1 mL (500 rxns) – RS31326-05
15 x 1 mL (1500 rxns) – RS31326-15
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.