HotStart DNA Polymerase, 5U/μL
HotStart DNA Polymerase is a mixture of anti-Taq monoclonal antibody and Taq DNA Polymerase, suitable for Hot Start PCR. When using Taq enzyme antibody for PCR amplification, the binding of Taq enzyme antibody to Taq enzyme inhibits DNA polymerase activity before high temperature denaturation, which can effectively inhibit the non-specific annealing of primers and non-specific amplification caused by primer dimers under low temperature conditions. The Taq enzyme antibody is neutral in the initial DNA denaturation step of the PCR reaction, and the DNA polymerase activity is restored to achieve the hot start effect. The use of this product does not require special inactivation of Taq enzyme antibody, and can be used under conventional PCR reaction conditions.
Applications
- Hot-start PCR amplification
- Specific amplification of complex cDNA and genomic template
- Amplification from low copy number DNA template
- Real-Time PCR
- Multiple PCR
- Generation of PCR products for TA cloning, Gene microarray analysis and SNP test
Components
| Component | HDP01 500 Units | HDP02 2500 Units |
| HotStart DNA Polymerase, 5U/μL | 100 μL | 5 × 100μL |
| 10× PCR Buffer | 1.8 mL | 5 x 1.8 mL |
Note: The 10×PCR Buffer of this product contains 15 mM magnesium ions.
Shipping and Storage
-20°C
Activity definition
Using activated salmon sperm DNA as template/primer, the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acidic insoluble material was defined as 1 active unit (U) at 74°C for 30 minutes.
Quality control
After several column purifications, the purity of SDS-PAGE test is more than 99%; no exogenous nuclease activity is detected; no host residual DNA is detected by PCR method; it can effectively amplify single-copy genes in the human genome
SKU: HDP01, HDP02
