Alkaline Phosphatase (Thermosensitive), 1000U

Product Description:

Alkaline Phosphatase (Thermosensitive) catalyzes the release of 5’- and 3’- phosphate groups from DNA, RNA, dNTP and rNTP. This enzyme also removes phosphate groups from proteins. 

Applications: 
Dephosphorylation of cloning vector DNA to prevent re-circularization during ligation. 
PCR product clean-up: nucleotide degradation prior to sequencing of PCR product. 
Dephosphorylation of nucleic acid 5’-termini prior to labeling with T4 Polynucleotide Kinase. 
 
Source: E.coli with a cloned bacterial AP gene form AntarcticBacteriumTAB5. Purity: ≥95% by SDS-PAGE
 
Molecular Weight: ~35 kDa
 
Concentration: 5 U/μL
 
Definition of Activity Unit: One unit is the amount of enzyme required to catalyze the dephosphorylation of 1 µg of linearized pUC19 DNA 5’ end in 10 minutes at 37 °C. Dephosphorylation is defined as greater than 95% inhibition of re-ligation of linearized plasmid DNA (as determined by E. coli transformation). 
 
10× ALP Reaction Buffer: 500 mM Bis-Tris-HCl, 10 mM MgCl2, 1 mM ZnCl2, pH 6.0. 
 
Storage (Dilution) Buffer: 10 mM Tris-HCl, 1 mM MgCl2, 0.01 mM ZnCl2, 50% Glycerol, pH 7.4. 
 
Inactivation or inhibition 
Inhibitors: metal chelators. 
Inactivated by heating at 80°C for 2 minutes.