Feyond-A400 Multi-Mode Microplate Reader, ABS / FL / LUM / TRF
The Feyond-A400 is a four-mode microplate reader that adds time-resolved fluorescence (TRF) to the absorbance, fluorescence and luminescence capabilities of the Feyond-A300. It suits labs running TRF immunoassays alongside routine ELISA, reporter-gene and cell-based assays on a single instrument.
- Four detection modes: absorbance (ABS), fluorescence (FL), luminescence (LUM) and time-resolved fluorescence (TRF).
- Sensitive TRF: detection limit as low as 0.02 pM under optimized conditions.
- Full spectral scanning: any wavelength from 200 to 1000 nm using a monochromator.
- Many plate formats: 6 to 384-well plates, PCR plates, and optional u-Nano ultra-micro plates.
- Incubation and shaking: up to 45 °C, with linear, circular and double-circular shaking.
- Upgradable: optional dual automatic injector and easy-change modular filters.
- Software: ReaderIt-II analysis software.
Catalogue number AS-19060-00.
Technical Specifications
- Model: Feyond-A400 (catalogue no. AS-19060-00), multi-mode: ABS, FL, LUM, TRF
- Plates: 6, 12, 24, 48, 96 and 384-well; 96/384 PCR plates; u-Nano 16/96 ultra-micro plates
- Absorbance: xenon lamp; monochromator 200-1000 nm in 1 nm steps; wavelength accuracy 2 nm, repeatability 0.2 nm, bandwidth under 2.5 nm; range 0-4 OD, resolution 0.0001 OD; accuracy ±(1.0% + 0.003 OD) up to 2.0 OD; linearity R² ≥ 0.999 (0-3 OD); stray light 0.1% at 220 nm; 96-well plate read in under 15 s (fast mode)
- Fluorescence: top reading; xenon lamp; PMT detector; excitation 200-1000 nm, emission 270-850 nm; standard filters EX485/EM530, EX523/EM564, EX624/EM692 (others available); detection limit 1 pM; linear dynamic range 6 logs or more
- Luminescence: PMT detector; detection limit 15 amol/well or better (96-well white plate, flash ATP); crosstalk 0.005% or lower; 200-850 nm; linear dynamic range 6 logs or more
- Time-resolved fluorescence (TRF): filter EX365/EM612; detection limit 0.02 pM (optimized conditions)
- Read types: endpoint, kinetics, spectral scanning, well scanning (up to 900 points per well)
- Shaking: linear, circular, double circular; speed and strength adjustable
- Incubation: room temperature + 4 °C up to 45 °C; ±0.5 °C at 37 °C
- Display: 10-inch capacitive touch screen; 10 GB data storage
- Software: ReaderIt-II PC analysis software (Windows 10 and above, 64-bit); test reports can be uploaded to a PC server by FTP
- Ports: USB A x2, USB B x1, Ethernet x1, RS232 (for injector)
- Optional automatic injector: 1 or 2 channels, 5-1000 µL in 1 µL steps, 125-500 µL/s, accuracy ±1 µL (5-50 µL) or ±2% (51-1000 µL), 25 mL waste collection
- Power: AC 100-240 V, 50-60 Hz
- Dimensions (W x D x H): 420 x 550 x 386 mm
- Weight: 33 kg
















