Mueller-Hinton Broth (MHB), Dehydrated Powder

Mueller-Hinton Broth (MHB) is a general-purpose liquid medium for growing a wide range of non-fastidious aerobic and facultatively anaerobic bacteria. Its low content of sulfonamide, trimethoprim and tetracycline inhibitors and its consistent batch-to-batch performance make it the medium most commonly used for broth-based antimicrobial susceptibility testing, such as minimum inhibitory concentration (MIC) assays.
 
Key features
Supports reliable growth of common Gram-negative and Gram-positive bacteria
Low levels of antimicrobial inhibitors, suited to MIC and susceptibility work
Starch absorbs toxic metabolites that could otherwise affect growth
Dehydrated powder: weigh, dissolve, autoclave
Available in 500 g, 1 kg and 5 kg
 
Applications
Broth microdilution and macrodilution MIC assays
Antimicrobial and antibiotic screening of new compounds
Routine cultivation of non-fastidious bacteria
Preparing inocula for susceptibility testing
 
Typical formula (per litre)
Beef extract: 2.0 g
Acid hydrolysate of casein: 17.5 g
Starch: 1.5 g
Final pH (25 °C): 7.3 ± 0.1
 
Preparation
Suspend 21 g of powder in 1 L of purified water.
Mix well and heat gently with stirring until fully dissolved.
Dispense as required and autoclave at 121 °C for 15 minutes.
Cool before use. Store prepared broth at 2–8 °C.
 
Storage
Store the dehydrated powder tightly closed at 15–25 °C in a dry place, protected from light. The powder is hygroscopic.
 
Ordering information
500 g: SKU MHB-500
1 kg: SKU MHB-1Kg
5 kg: SKU MHB-5Kg
 
For research use only.