PuroSPIN™ NanoElute™ ccfDNA Purification Kit
PuroSPIN™ NanoElute™ ccfDNA Purification Kit is designed for purification of circulating DNA from plasma or serum sources. Plasma/Serum Cell-Free Circulating DNA (cfc-DNA) has emerged as a valuable source of biomarkers for cancer, autoimmune diseases, and prenatal testing. Advances in this field are enabling its use in early diagnosis, prognosis, and therapy monitoring for various cancers and immune-related conditions. Cell-free mitochondrial DNA (cfmtDNA) is also being explored for its clinical relevance. Typically, cfc-DNA exists as short fragments under 1000 bp. In prenatal applications, cell-free fetal DNA is widely used for non-invasive testing, including fetal sex determination, genetic screening in high-risk families, Rhesus factor typing, aneuploidy detection, and early identification of preeclampsia.
Features:
- Easily purify 200 μL of plasma/serum samples using silica spin columns
- Simple extraction process with no need for phenol or chloroform
- Specialized and detailed protocols for different volumes of a sample
- High-quality extracted ccfDNA can be used for a range of downstream applications such as genomic sequencing, PCR, real-time qPCR, or Southern blotting
SKU List: #NK367-5, #NK367-50, #NK367-250
Product Manual
Product Specifications
Kit Type: ccfDNA Purification Kit Format: Silica Spin Columns Kit Scale: NANO Starting Material: Plasma or serum Sample Volume: 200 μL Processing Time: < 45 minutes Elution Volume: 6 - 20 μL Typical Yield: Varies Downstream Applications: Ligation, microarray analyses, qPCR, RT-qPCR, sequencing, Southern blotting, transfection, transformation, etc.
