PuroSPIN™ Total RNA Blood Purification Kit (NK052-50, NK052-250)

The PuroSPIN™ Total RNA Blood Purification Kit is designed to purify total RNA from blood. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns. The nucleic acid is first released form the blood by the treatment with the Lysis Buffer LB-B. RNA is then loaded onto silica-based spin columns and washed to remove protein, salt, and other contaminants. An on-column DNase I digestion in- between wash steps ensures the removal of any contaminating DNA. Finally, purified RNA is eluted from the column into a clean microcentrifuge tube in nuclease-free water.

High quality RNA extracted with the PuroSPIN™ Total RNA Blood Purification Kit can be used for a range of downstream applications such as reverse-transcription qPCR, Northern blotting, or microarray analysis.
 
Features:
Extract total RNA from whole blood, serum and plasma samples
Simple silica-spin columns extraction process with no need for phenol or chloroform
Includes on-column DNase I digestion step to remove contaminating DNA
High quality extracted RNA can be used for a range of downstream applications such as cDNA library construction, microarray analyses, Northern blotting, qPCR, and RT-qPCR
 
SKU List: #NK052-50, #NK052-250