PuroSPIN™ Fibrous Tissue RNA Purification Kit #NK053-50, #NK053-250

PuroSPIN™ Fibrous Tissue RNA Purification Kitis designed for extraction of total RNA from fibrous animal tissue, such as skin, skeletal muscle, lung, or heart. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns. The tissue is first disrupted through homogenization and Proteinase K treatment. The RNA is then released form the cells by the treatment with the Lysis Buffer LB-R. Following the lysis step, RNA is loaded onto silica-based spin columns and washed to remove protein, salt and other contaminants. The kit includes on-column DNase I digestion step to remove any contaminating DNA. Finally, purified RNA is eluted from the column into a clean microcentrifuge tube in Elution Buffer EB, or nuclease-free water.
 
The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatments. High quality RNA extracted with the PuroSPIN™ Fibrous Tissue RNA Purification Kitcan be used for a range of downstream applications, such as reverse-transcription PCR or qPCR, northern blotting, microarray analysis.
 
For purification of RNA from soft tissue, such as spleen, kidney, liver, or brain, or from bacteria, yeast, or mammalian cells, please use our PuroSPIN™ Total RNA Purification Kit(#NK051-50, #NK051-250).
 
PuroSPIN™ columns can purify up to 200 μg of RNA product.
 
Features:
Extract total RNA from a variety of fibrous tissue samples
Simple extraction process with no need for phenol or chloroform 
Includes on-column/on-bead DNase I digestion step to remove contaminating DNA
High quality extracted RNA can be used for a range of downstream applications such as cDNA library construction, microarray analyses, Northern blotting, qPCR, and RT-qPCR
 
Citations:
laura M. Androschuk, Theresa H. Tam, Redouan Mahou, Cheun Lo, Michael W. Salter, Michael V. Sefton. Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of mice. Biomaterials, Volume 289, 2022, 121764, ISSN 0142-9612, https://doi.org/10.1016/j.biomaterials.2022.121764.
Ngo, W., Wu, J.L.Y., Lin, Z.P. et al. Identifying cell receptors for the nanoparticle protein corona using genome screens. Nat Chem Biol 18, 1023–1031 (2022).
 
SKU List: NK053-50, NK053-250