PuroMAG™ Fibrous Tissue RNA Purification Kit

The PuroMAG™ Fibrous Tissue RNA Purification Kit is designed to purify total RNA from fibrous animal tissue, such as skin, skeletal muscle, lung or heart, using silica-coated magnetic beads.
RNA binds to silica-coated magnetic beads, allowing efficient removal of genomic DNA, protein, salt and other contaminants. Silica-based purification provides a faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. The kit includes a DNase I digestion step to remove any contaminating DNA.
Key features
Extract total RNA from a variety of fibrous tissue samples
Flexible magnetic bead-based protocols (low sample number manual purification or high-throughput automated purification); centrifugation is not required
Includes an on-column/on-bead DNase I digestion step to remove contaminating DNA
Can also be used for RNA cleanup after DNase I or other enzymatic treatments
Manual and automated processing
The high-throughput protocols have been optimized for Auto-Pure Nucleic Acid Purification systems but can be adapted for other purification systems (e.g. KingFisher Flex, Hamilton Microlab STAR, Biomek FX). The PuroMAG™ 8-Hole Mini Magnetic Rack (Cat# MGR-008) and PuroMAG™ 16-Hole Mini Magnetic Rack (Cat# MGR-016) are well suited to fast and convenient manual purification of RNA.
Applications
High-quality RNA extracted with this kit can be used for downstream applications such as reverse-transcription qPCR, Northern blotting or microarray analysis.
For RNA from soft tissue, such as spleen, kidney, liver or brain, or from bacteria, yeast or mammalian cells, please use our PuroMAG™ Total RNA Purification Kit (#NKM051-96, #NKM051-384).
Available sizes
96 preps – NKM053-96
384 preps – NKM053-384
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.