PuroSPIN™ Soil RNA Purification Kit

The PuroSPIN™ Soil RNA Purification Kit is a silica spin-column soil RNA extraction kit for purifying total RNA from a variety of microorganisms in soil samples, including fungi, bacteria and archaea.
It simplifies purification with a convenient extraction technology based on nucleic acid binding to silica spin columns, eliminating toxic phenol-chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for different soil types, such as top-soil, bog, clay, forest and compost.
Performance
Up to 500 mg of soil or sediment per sample can be processed in less than 1 hour. RNA yield typically ranges from 1 to 5 µg, varying between soil types, with an A260/280 ratio of 1.9–2.1 and an A260/230 ratio of ≥1.9, indicating a high-quality product. Purified RNA can be used for downstream applications such as reverse-transcription qPCR, Northern blotting or microarray analysis.
Key features
Easily purify RNA from soil samples using silica spin columns
Simple extraction process with no need for phenol or chloroform
Lysate filtration step removes precipitated RT-PCR inhibitors, genomic DNA, SDS-protein complexes, salts and other contaminants for higher RNA yield and purity
Specialized, detailed protocols for different sample types
High-quality RNA for downstream applications such as RT-PCR and real-time qPCR
Available sizes
5 preps – NK056-5
50 preps – NK056-50
200 preps – NK056-200
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.