PuroSPIN™ Soil DNA MAXI Purification Kit
The PuroSPIN™ Soil DNA MAXI Purification Kit is a soil DNA extraction kit designed to purify high molecular weight genomic DNA from up to 10 grams of soil, from a variety of microorganisms including fungi, bacteria and archaea.
The kit simplifies purification with a convenient, easy-to-use extraction technology based on nucleic acid binding to silica spin columns, eliminating toxic phenol-chloroform extraction and time-consuming alcohol precipitation. It is suitable for different types of soil, such as top-soil, bog, clay, forest and compost.
Key features
- Easily purify genomic DNA from up to 10 grams of soil using silica spin columns
- Simple extraction process with no need for phenol or chloroform
- Lysate filtration step removes precipitated PCR inhibitors, SDS-protein complexes, salts and other contaminants for higher yield and purity of genomic DNA
- Suitable for top-soil, bog, clay, forest, compost and other soil types
Applications
High-quality extracted genomic DNA can be used for downstream applications such as genomic sequencing, PCR, real-time qPCR or Southern blotting.
Available sizes
- 2 prep sample – NK163-2
- 10 preps – NK163-10
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.
Product Manuals
Product Data Sheet
Product Specifications
Kit Type: Soil Genomic DNA Extraction/Purification Kit Format: Silica Spin Columns Kit Scale: MAXI Starting Material: Top-soil, bog, clay, forest, compost and other types of soil Processing Time: < 1 hour Typical Yield: 50 to 200 ug Elution Volume: 3 mL Ratio A260/A280: 1.7 - 1.9 Ratio A260/A230: 1.8 - 2.2 Downstream Applications: Ligation, microarray analyses, qPCR, RT-qPCR, sequencing, Southern blotting, transfection, transformation, etc.
