PuroSPIN™ NanoElute™ Genomic DNA Purification Kit
The PuroSPIN™ NanoElute™ Genomic DNA Purification Kit is a low-elution-volume genomic DNA kit for bacteria, yeast, mammalian cells, blood and animal tissue. The protocol releases genomic DNA from cells and purifies it from protein, salt and other contaminants, while highly efficient RNase and optimized buffers ensure efficient removal of contaminating RNA.
Compared with our standard PuroSPIN™ Genomic DNA Purification Kit, the NanoElute version uses Nano silica spin columns instead of mini columns. Nano columns provide the highest possible concentration of purified genomic DNA, with elution volumes as low as 6 µL.
The NanoElute kit is particularly well suited to very small amounts of starting material, as adding Carrier DNA to the procedure allows successful extraction and processing of such samples.
Key features
- Easily purify genomic DNA using silica spin columns
- Simple extraction with no need for phenol or chloroform
- Unique Nano silica spin columns maximize the concentration of DNA purified from small samples
- Specialized, detailed protocols for different sample types
- High-quality genomic DNA for genomic sequencing, PCR, real-time qPCR or Southern blotting
Available sizes
- 5 preps – NK361-5
- 50 preps – NK361-50
- 250 preps – NK361-250
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.
Materials Safety Data Sheets (MSDS)
Product Data Sheet
Product Specifications
Kit Type: Genomic DNA Extraction/Purification Kit Format: Silica Spin Columns Kit Scale: NANO Starting Material: Bacteria, yeast, mammalian cultured cells, animal tissue, and whole mammalian blood Processing Time: < 45 minutes Processing Mode: Manual, Centrifugation/Vacuum Elution Volume: 6 - 20 μL Ratio A260/A280: 1.7 - 1.9 Ratio A260/A230: 1.9 - 2.2 Downstream Applications: Ligation, microarray analyses, qPCR, RT-qPCR, sequencing, Southern blotting, transfection, transformation, etc.
