PuroSPIN-96™ Genomic DNA Purification Plus Kit, 96-Well Plate Format, 384 Preps

The PuroSPIN-96™ Genomic DNA Purification Plus Kit is a 96-well genomic DNA purification kit (384 preps) for purifying genomic DNA from biological samples such as bacteria, yeast, mammalian cells, blood and animal tissue using 96-well silica membrane purification plates.
Genomic DNA is released from cells or tissue using Lysis Buffer LB-GD1, Lysis Buffer LB-GD2 and Proteinase K. The DNA is then loaded onto silica-based purification plates and washed to remove protein, salt and other contaminants. Finally, the purified DNA is eluted into a clean 96-well elution plate in Elution Buffer EB or nuclease-free water.
What the Plus kit adds
Compared with our PuroSPIN-96™ Genomic DNA Purification Kit, the Plus kit adds a 96-well plate filtration step that removes the insoluble and non-digested fraction from the lysate before it is loaded onto the 96-well silica spin plate. This prevents contaminating material from binding to and blocking parts of the silica membrane, leading to increased genomic DNA yield and higher purity. It is particularly beneficial for large samples with a lot of starting material to digest.
High-quality DNA extracted with the PuroSPIN-96™ Genomic DNA Purification Plus Kit can be used for downstream applications such as PCR, Southern blotting or genomic sequencing.
NOTE: Our Universal Vacuum Manifold for 96-Well Plates or Double-Layer Vacuum Manifold for 96-Well Plates can ONLY be used with the Semi-Skirted 96-well Nucleic Acid Purification Plate.
Key features
Easily purify genomic DNA from a variety of biological samples
High-throughput sample processing using 96-well silica membrane purification plates
Additional 96-well plate filtration step removes the insoluble and non-digested fraction for improved yield and higher purity
Simple extraction process with no need for phenol or chloroform
Specialized, detailed protocols for different sample types
High-quality genomic DNA for genomic sequencing, PCR, real-time qPCR or Southern blotting
Available plate formats (384 preps)
Full-Skirted plate – NKP261-384
Semi-Skirted plate – NKPS261-384
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.