PuroSPIN™ Plasmid MIDIprep Plus Kit

The PuroSPIN™ Plasmid MIDIprep Plus Kit is a silica spin-column plasmid midiprep kit for extracting up to 200 µg of plasmid DNA from up to 100 mL of overnight bacterial culture.
Plasmid DNA binds to silica spin columns, allowing efficient removal of genomic DNA, protein, salt and other contaminants. Silica spin columns offer a faster, simpler and safer alternative to traditional techniques such as phenol-chloroform extraction, and the PuroSPIN™ buffer formulations produce highly purified plasmid DNA for transfection, enzymatic digestion or PCR.
Key features
Purify plasmid DNA from up to 100 mL of overnight bacterial culture
Plus kit includes a syringe filter that quickly removes the insoluble fraction from the plasmid preparation, eliminating the 12,000 x g centrifugation step
Extraction of up to 200 µg of plasmid DNA
Rapid purification of plasmid DNA (<60 minutes), compared with ion-exchange column formats (>5 hours)
Efficient removal of genomic DNA, protein, salt and other contaminants
High-quality plasmid DNA for cloning, PCR, restriction enzyme digestion, sequencing, transformation or transfection
Endotoxin-free formats also available
Available sizes
2 preps trial kit – NK212-2
20 preps – NK212-20
50 preps – NK212-50
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.