PuroSPIN™ Plasmid Miniprep Plus Kit #NK211-5, #NK211-50, #NK211-200

The PuroSPIN™ Plasmid Miniprep Plus Kit is designed to provide a simple and easy-to-use method for extraction and purification of plasmid DNA from cultured bacterial cells. The PuroSPIN™ buffer formulations produce highly purified plasmid DNA for downstream applications. 
 
In comparison to our standard PuroSPIN™ Plasmid Miniprep Kit, the Plus kit adds an additional filtration step that removes the precipitated SDS-protein complexes, genomic DNA, carbohydrates and other contaminants from the lysate before loading it onto the silica spin column. This prevents contaminating materials from binding to and blocking parts of the silica membrane, leading to an increased plasmid DNA yield and higher purity of the final product. This is particularly benficial when workign with large samples that contain a lot of starting material that needs to be processed.
 
Features:
 
Easily purify plasmids from bacterial cultures using silica spin columns
Simply extraction process with no need for phenol or chloroform
Optimized buffer formulations to ensure efficient removal of RNA from plasmid samples
Lysate filtration step to remove any precipitated SDS-protein complexes, genomic DNA, salts and other contaminants for higher yield and purity plasmid DNA products
High quality extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing transformation, or transfection
 
 
SKU List: NK211-5, NK211-50, NK211-200