PuroSPIN™ Endo-Free Plasmid Miniprep Kit

The PuroSPIN™ Endo-Free Plasmid Miniprep Kit is a silica spin-column endotoxin-free plasmid miniprep kit for simple extraction and purification of plasmid DNA from cultured bacterial cells. It extracts up to 15 µg of plasmid DNA with endotoxin levels <0.1 EU/µg.
Plasmid DNA binds to silica spin columns, allowing efficient removal of genomic DNA, protein, salt and other contaminants. Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB. Silica spin columns offer a faster, simpler and safer alternative to traditional techniques such as phenol-chloroform extraction, and the PuroSPIN™ buffer formulations produce highly purified plasmid DNA for transfection, enzymatic digestion or PCR.
Key features
Purify endotoxin-free plasmid DNA from small volumes of bacterial culture
Extract up to 15 µg of highly pure plasmid DNA with endotoxin levels <0.1 EU/µg
Simple extraction process with no need for phenol or chloroform
Optimized buffer formulations ensure efficient removal of RNA from plasmid samples
Efficient removal of genomic DNA, protein, salt and other contaminants
High-quality plasmid DNA for cloning, PCR, restriction enzyme digestion, sequencing, transformation or transfection
Available sizes
5 preps trial kit – NKE111-5
50 preps – NKE111-50
250 preps – NKE111-250
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.