Benzamidine AgaDex 4FF (HS) Affinity Resin
Product Description
Benzamidine AgaDex 4FF (HS) Affinity Resin is designed for the separation and purification of serine proteases such as pancreatic protease, thrombin, enterokinase, urinary kallikrein, and prolyl endopeptidase (peptidase-releasing enzyme) using benzamidine, a serine protease inhibitor as a ligand. It also enables rapid removal of cell culture supernatants, bacterial lysates, and serine proteases from serum, making it highly effective for diverse sample preparation workflows.
Product Features
- Rapid and simple (one-step purification)
- Offers a higher binding capacity for serine proteases, allowing more target protein to be captured per milliliter of resin.
- Contains a higher concentration of immobilized benzamidine ligands.
- Allows for greater productivity and higher protein yields, especially when purifying large quantities of protein.
- Ideal for large-scale purification or for samples with high concentrations of serine proteases.
Storage
Store at 4-8 °C. Valid for 12 months.
Technical Parameters
Resin Highly crossed-linked 4% agaroseParticle Size Range 45-165 µm Average Particle Size 90 ± 5 µm Binding Capacity ≥30 mg/ml pancreatic proteasepH Stability 1-9 (Working) 2-8 (Cleaning)Chemical Stability Compatible with commonly used buffer solutions, 8M urea, 6M guanidine HClLinear Flow Velocity (0.3 MPa) ≥250 cm/h Operating Pressure ≤0.3 MPa Sotrage Solution 50mM NaAc in 20% Ethanol pH 4.0Storage Temperature 4-8 °C
