Ni-NTA AgaDex FF Affinity Resin
Product Description
Ni-NTA FF AgaDex Affinity Resin is suitable for the separation and purification of His-tag proteins and other biomolecules that interact with Ni2+.
Product Features
- Fast & Simple Purification
- Suitable for gravity columns and prepacked columns, including systems using peristaltic pumps or chromatography setups.
- Versatile Chelation Options: Supports a range of metal ions—such as Cu²⁺, Zn²⁺, Fe²⁺, Co²⁺, and Ca²⁺ to accommodate diverse purification needs.
Note: When using Ca²⁺ as the chelating ion, avoid phosphate buffers to prevent precipitation.egeneration.
Storage
Store at 4-30 °C, valid for 12 months.
Technical Parameters
Resin Highly corss-linked 6% agaroseParticle Size Range 45-165 µm Average Particle Size 90 ± 5 µm Binding Capacity ≥40mg (His-tag protein)/ml resinMetal Ion Loading ≥16-23 µmole (Ni+2)/ml resinCoordination Ratio (NTA:Ni+2) 4:2 pH Stability 3-12 (working) 2-14 (cleaning)Chemical Stability Avoid using chelating agents such as EDTA and EGTA at concentration higher than 1mM, and redusing agents such as DDT and DTE at concentrations higher than 5mM.Linear Flow Velocity (0.3 MPa) ≥300 cm/h Operating Pressure ≤0.3 MPa Sotrage Solution 20% Ethanol Storage Temperature 4-30 °C Scope application Routine His-tagged protein purification (active and denatured conditions)
