Ni-IDA AgaDex FF Affinity Resin
Product Description
Ni-IDA FF Agadex Affinity Resin utilizes interaction between Ni²⁺ ions and amino acid side chains primarily histidine, cysteine, and tryptophan to enable targeted separation and purification. Ideal for isolating His-tagged proteins and other biomolecules with affinity for Ni²⁺.
Product Features
- Fast & Simple Purification
- Suitable for gravity columns and prepacked formats, including systems using peristaltic pumps or chromatography setups.
- Versatile Chelation Options: Supports a range of metal ions such as Cu²⁺, Zn²⁺, Fe²⁺, Co²⁺, and Ca²⁺ to accommodate diverse purification needs.
- Note: When using Ca²⁺ as the chelating ion, avoid phosphate buffers to prevent precipitation.egeneration.
Storage
Store at 4-30 °C, valid for 12 months.
Technical Parameters
Resin Highly cross-linked 6% agaroseAverage Particle Size 90 ± 5 µm Particle Size Range 45-265 µm Binding Capacity ≥30mg (His-tag protein)/ml resinMetal ion loading ≥30 µmole (Ni+2)/ml resinCoordination Ratio (IDA: Ni+2) (3:3) pH Stability 3-12 (working) 2-14 (cleaning)Chemical Stability Avoid using chelating agents such as EDTA and EGTA, and redusing agents such as DDT and DTELinear Flow Velocity (0.3 MPa) ≥300 cm/h Operating Pressure ≤0.3 MPa Sotrage Solution 20% Ethanol Storage Temperature 4-30 °C Application scope Conventional His-tagged protein purification (active condition)
