Ni-TED AgaDex FF Affinity Resin
Product Description
Ni-TED AgaDex FF Affinity Resin is designed for the purification of His-tagged proteins and other biomolecules with affinity for Ni²⁺ ions. Its strong chelating properties enable direct application in purifying His-tagged proteins expressed in eukaryotic secretion systems, withstanding elevated levels of reducing agents and chelators eliminating the need for sample pretreatment. The resin can be easily cleaned and regenerated without nickel stripping, enabling direct NaOH cleaning.
Product Features
- Fast and simple (one-step purification)
- Tolerates higher levels of reducing agents and chelators, allowing direct application of His-tagged proteins expressed in eukaryotic secretion systems without pretreatment, maximizing protein activity preservation.
- No need for nickel stripping, allowing direct NaOH cleaning, significantly shortening the cleaning cycle.
- Lower nickel leaching compared to traditional NiIDA AgaDex Resin and Ni-NTA AgaDex Resin, eliminating the need for repetitive regeneration.
Storage
Store at 4-30 °C, valid for 12 months.
Technical Parameters
Resin Highly cross-linked 6% agaroseParticle Size Range 45-165 µm Average Particle Size 90 ± 5 µm Binding Capacity ≥10mg (His-tag protein)/ml resinCoordination Ratio (TED:Ni+2) 5:1 pH Stability 3-12 (working) 2-14 (cleaning)Chemical Stability 10mM HCl, 10mM NaOH, 8M urea, 6M guanidine HCl (24 hrs), 100mM EDTA, 0.5M imidazole (2 hrs). 30% isopropanol (20 mins), 20mM DDTLinear Flow Velocity (0.3 MPa) ≥300 cm/h Operating Pressure ≤0.3 MPa Sotrage Solution 20% Ethanol pH 4.0Storage Temperature 4-30 °C Scope Application Can be used for high reductant, chelator His-tagged protein samples and eukaryotic His-tagged protein purification
