Poly-D-Lysine Solution, 10X, Sterile, for Cell Culture Coating

A sterile 10X poly-D-lysine solution for coating culture surfaces so adherent cells attach and spread more readily. Dilute it to 1X before use.
How it works
Poly-D-lysine is a positively charged synthetic polymer that coats glass or plastic and attracts the negatively charged cell membrane. Its D-form is not recognised by cell proteases, so cells cannot break the coating down, which makes it a common choice for neurons and other cells grown for long periods.
Key features
Sterile 10X concentrate
Dilute to a 1X coating solution before use
5 mL of concentrate gives 50 mL of 1X working solution
How to use
Dilute the 10X solution 1:10 with sterile water or buffer.
Cover the culture surface with the 1X solution and leave it to coat.
Remove the solution, rinse if your protocol requires it, let the surface dry, then seed the cells.
Specifications
Type: Poly-D-lysine solution, 10X
Sterility: Sterile
Working strength: 1X after 1:10 dilution
Catalog number: LN-G4031-5ML