Poly-L-Lysine Solution, 10X, Sterile, for Cell Culture Coating
A sterile 10X poly-L-lysine solution for coating culture surfaces so adherent cells attach and spread more readily. Dilute it to 1X before use.
How it works
Poly-L-lysine is a positively charged polymer. Coated onto glass or plastic, it gives the surface a positive charge that attracts the negatively charged cell membrane, which improves attachment for weakly adherent cells and for cells grown on glass.
Key features
- Sterile 10X concentrate
- Dilute with water or buffer to a 1X coating solution
- 10 mL of concentrate gives 100 mL of 1X working solution
How to use
- Dilute the 10X solution 1:10 with sterile water or buffer.
- Cover the culture surface with the 1X solution and leave it to coat.
- Remove the solution, rinse if your protocol requires it, let the surface dry, then seed the cells.
Specifications
- Type: Poly-L-lysine solution, 10X
- Sterility: Sterile
- Working strength: 1X after 1:10 dilution
- Catalog number: LN-G4025-10ML

