Antibody Purification Kit, Protein A Agarose, 10 Preps

A complete Protein A agarose kit for purifying monoclonal and polyclonal antibodies and Fc-fusion proteins from serum, ascites or culture supernatant by gravity flow. Buffers and empty columns are included.
5 mL Protein A agarose (50% slurry), binding over 1 mg mouse antibody per mL
Glycine pH 2.8 acid elution with 1 M Tris pH 9.0 neutralization
Five empty 12 mL gravity columns included
10X PBS and 5X loading buffer included for binding and checking fractions
Components
Protein A agarose 5 mL (50% slurry); 10X PBS pH 7.4 100 mL; elution buffer 2 × 75 mL (100 mM glycine pH 2.8); neutralization buffer 15 mL (1 M Tris pH 9.0); 5X loading buffer 1 mL; 5 empty 12 mL columns; manual
Not included
Desalting or dialysis columns, protease inhibitors, filters
Preps
10
Catalogue number
GLN2246-10T
For research use only. Not for use in diagnostic or therapeutic procedures.