PuroMAG™ Protein G Coated Magnetic Beads

PuroMAG™ Protein G coated magnetic beads are designed for efficient capture of IgG and other antibodies from serum, cells and other biological samples, and for immunoprecipitation.
Protein G is a cell wall component produced by group G Streptococcus strains. It binds the Fc part of a wide range of immunoglobulins (Igs), so Protein G coupled to PuroMAG superparamagnetic beads is suitable for fast and efficient capture of a wide range of Igs. Protein G shows different affinity for Igs from different species and for different isotypes within a species (see the Ig binding strength table in the info section).
How it works
The sample containing Igs is first mixed with PuroMAG Protein G coated beads. During a 30-minute incubation, the Igs bind to the PuroMAG-PRG beads via their Fc region. The samples are then placed on a magnetic rack for pull-down and washing, producing a purified complex of antibodies bound to Protein G beads. Bound antibodies can then be covalently cross-linked to bead-immobilized Protein G to give a very stable bead-antibody complex for antigen capture experiments.
A strong magnetic core allows PuroMAG™ magnetic beads to be separated from solution with magnets in under 30 seconds, enabling easy processing on magnetic racks without a centrifuge. PuroMAG™ magnetic bead technology can also be used with automated extraction systems for high-throughput nucleic acid purification.
Key features
High antibody binding capacity: IgG binding capacity of 39–41 µg/100 µL beads
Rapid and efficient IgG purification from complex samples
Fast response to a magnet
High monodispersity
Applications
Magnetic separation of antibodies: the Protein G coated magnetic beads are simply added to antibodies for binding and capture
During incubation, the antibody binds to the MGB-PRG beads via its Fc region; samples are transferred to a magnetic rack for easy removal of unbound residue
The bead-bound antibody can be used for immunoprecipitation
At Luna Nanotech we use the power of nanotechnology to optimize and improve the quality and surface binding capacity of nano- and micromaterials. Try our magnetic beads – we are confident they will outperform the competition!
Available sizes
1 mL – MGB-PRG-1
5 mL – MGB-PRG-5
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.