PuroMAG™ Protein L Coated Magnetic Beads

PuroMAG™ Protein L Coated Magnetic Beads are protein L magnetic beads designed for efficient capture of IgG and other antibodies from serum, cells and other biological samples.
Protein L coupled PuroMAG superparamagnetic beads are suitable for fast and efficient capture of a wide range of Igs. Protein L shows varying affinity for Igs from different species and for different isotypes within a species (see the Ig binding table in the info section).
How it works
The sample containing Igs is first mixed with PuroMAG Protein L coated beads. During a 30-minute incubation the Igs bind to the PuroMAG-PRL beads. The samples are then placed on a magnetic rack for pull-down and washing, producing a purified complex of antibodies bound to Protein L beads. Bound antibodies can then be covalently cross-linked to the bead-immobilized Protein L to give a very stable bead-antibody complex for further antigen capture experiments.
The strong magnetic core allows PuroMAG™ Magnetic Beads to be separated from solution with a magnet in under 30 seconds, enabling easy processing with magnetic racks and removing the need for a centrifuge. PuroMAG™ Magnetic Bead technology can also be used with automated extraction systems for high-throughput nucleic acid purification.
At Luna Nanotech we use the power of nanotechnology to optimize and improve the quality and surface binding capacity of nano- and micromaterials. Try our magnetic beads – we are confident they will outperform our competitors!
Key features
High antibody binding capacity: IgG binding capacity 53–70 µg/100 µL beads
Rapid and efficient IgG purification from complex samples
Fast response to a magnet
High monodispersity
Applications
Magnetic separation of antibodies: Protein L coated magnetic beads are simply added to antibodies for binding and capture
After incubation, samples are transferred to a magnetic rack for easy removal of unbound residue
Bead-bound antibodies can be used for immunoprecipitation
Available sizes
1 mL – MGB-PRL-1
5 mL – MGB-PRL-5
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.