PuroMAG™ Protein A/G Coated Magnetic Beads

PuroMAG™ Protein A/G Coated Magnetic Beads are protein A/G magnetic beads designed for efficient capture of IgG and other antibodies from serum, cells and other biological samples.
Protein A/G-coupled PuroMAG superparamagnetic beads are suitable for fast and efficient capture of a wide range of immunoglobulins. Protein A/G shows varying affinity for Ig's from different species and for different isotypes within a species (see the Ig binding table in the info section).
How it works
The sample containing Ig's is mixed with PuroMAG Protein A/G beads. During a 30 min incubation the Ig's bind to the PuroMAG-PRAG beads via their Fc region. Samples are then placed on a magnetic rack for pull-down and washing, producing a purified complex of antibodies bound to Protein A/G beads. Bound antibodies can then be covalently cross-linked to the bead-immobilized Protein A/G to create a very stable bead-antibody complex for further antigen capture experiments.
A strong magnetic core allows PuroMAG™ Magnetic Beads to be separated from solution with a magnet in under 30 seconds, enabling easy processing with magnetic racks and removing the need for a centrifuge. PuroMAG™ magnetic bead technology can also be used with automated extraction systems for high-throughput nucleic acid purification.
At Luna Nanotech we use the power of nanotechnology to optimize and improve the quality and surface binding capacity of nano- and micromaterials. Try our magnetic beads – we are confident they will outperform our competitors!
Key features
High antibody binding capacity – IgG binding capacity: 41–43 µg / 100 µL beads
Rapid and efficient IgG purification from complex samples
Fast response to a magnet
High monodispersity
Applications
Magnetic separation of antibodies – the coated beads are simply added to antibodies for binding and capture
Antibodies bind the MGB-PRAG beads via their Fc region during incubation; samples are transferred to a magnetic rack for easy removal of unbound residue
Bead-bound antibodies can be used for immunoprecipitation
Available sizes
1 mL – MGB-PRAG-1
5 mL – MGB-PRAG-5
Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.