PuroSPIN™ PCR Product Purification Kit  #NK105-50, #NK105-250

 

  • PuroSPIN™ PCR Purification Kit is designed to provide simple and easy-to-use method for purification of PCR DNA products (70 bp − 10 kb) from enzymatic reactions.
  • The purification technology is based on nucleic acid binding to silica spin-columns, which allows efficient removal of protein, salt and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
  • PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as sequencing, ligation, transfection, Southern blotting, qPCR, or microarray analysis.
  • PuroSPIN™ columns can bind up to 200 μg of DNA product.
  • DNA purified by PuroSPIN™ PCR Product Purificaiton Kit shows equal or higher yield and purity (as determined by the 260/280 and 260/230 ratios) when compared to leading competitors in the field (see head-to-head comparison data in the "Competitor Kit Comparison" section on the right).

 

 

 

 

 

SKU List:

NK105-50, NK105-250

PuroSPIN™ PCR Product Purification Kit #NK105-50, #NK105-250

C$70.00Price
Size
  • Product Specifications

    Recovered DNA Size 70 bp – 10 kb
    DNA Recovery  90 – 95 %
    Column Binding Capacity 200 µg
    Column Reservoir Capacity 800 µL
  • Competitor Kit Comparison

    Data shown below compared purificaiton efficiency and purity of the product produced by the PuroSPIN™ PCR Product Purificaitn Kit and leading competitor kits. For these experiments PCR product was generated using standard 100 μL scale reaction. 10 μL of this PCR product was used for the tests. 100 bp, 150 bp, and 1500 bp PCR products were tested. 

     

    Table 1: Comparison of PCR product purification kits: 100 bp product

     

    PuroSPIN PCR Kit

    Comp. Q PCR Kit Comp. B PCR Kit  
    Yield (μg) 1.4 (±0.2) 1.0 (±0.1) 0.8 (±0.3)
    260/280 3.0 (±0.6) 2.2 (±0.1) 2.7 (±1.8)
    260/230 3.3 (±0.4) 0.2 (±0.1) 0.9 (±0.4)

    * Data based on 3 replicates. Numbers in brackets are standard deviations.

     

    Table 2: Comparison of PCR product purification kits: 150 bp product

     

    PuroSPIN PCR Kit

    Comp. Q PCR Kit    Comp. B PCR Kit   
    Yield (μg) 1.3 (±0.1) 1.1 (±0.1) 1.0 (±0.1)
    260/280 3.0 (±0.4) 2.3 (±0.3) 1.8 (±0.2)
    260/230 2.9 (±0.3) 0.1 (±0.1) 0.8 (±0.2)

    * Data based on 3 replicates. Numbers in brackets are standard deviations.

     

    Table 3: Comparison of PCR product purification kits: 1500 bp product

     

    PuroSPIN PCR Kit

    Comp. Q PCR Kit    Comp. B PCR Kit   
    Yield (μg) 3.8 (±0.1) 2.6 (±0.3) 4.2 (±0.1)
    260/280 1.7 (±0.1) 1.9 (±0.1) 1.8 (±0.1)
    260/230 1.8 (±0.1) 0.2 (±0.1) 1.0 (±0.9)

    * Data based on 2 replicates. Numbers in brackets are standard deviations.

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