Recombinant Larimichthys crocea Hepcidin (hamp)

Shipping Cost Note
Each Recombinant Protein price includes shipping cost to Canada and import fees. Please ask for a discounted quote if ordering multiple Cusabio products.
 
Delivery Time Note
This product is usually imported from the manufacturer after order placement. Please expect longer delivery times of 7-14 days.
 
Catalog No.
CSB-EP010124LLH
 
Product Name
Recombinant Larimichthys crocea Hepcidin (hamp)
 
In Stock
Yes
 
Activity
Not Tested
 
Research Area
Immunology
 
Uniprot ID
A1Z0M0
 
Gene Names
hamp
 
Alternative Names
hamp; Hepcidin
 
Organism
Larimichthys crocea (Large yellow croaker) (Pseudosciaena crocea)
 
Source
E.coli
 
Expressed Region
65-85aa
 
Protein Length
Full Length of Mature Protein
 
Tag Info
Tag-Free
 
Target Protein Sequence
RCRFCCRCCPRMRGCGICCRF
 
MW
2.5 kDa
 
Purity
Greater than 90% as determined by SDS-PAGE.
 
Endotoxin
Not Tested.
 
Form
Liquid or Lyophilized powder
 
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
 
Resonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
 
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. 
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
 
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
 
Relevances
Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages
Has very strong antibacterial activity against the marine Gram-negative bacteria V.alginolyticus (MIC=24 µM), V.fluvialis, V.harveyis (MIC=12 µM) and V.parahaemolyticus (MIC=6 µM). Has antibacterial activity against the Gram-negative bacteria A.hydrophila (MIC=6 µM), E.coli (MIC=24 µM), and E.coli BL21(DE3)plysS (MIC=6 µM), and the Gram-positive bacteria B.cereus (MIC=24 µM), B.subtilis (MIC=6 µM), C.glutamicum (MIC=3 µM), M.luteus (MIC=3 µM), M.lysodeikticus, S.aureus (MIC=6 µM) and S.epidermis (MIC=12 µM). Possesses antifungal activity against A.niger (MIC=24 µM), F.graminearum (MIC24 µM) and F.solani (MIC=24 µM), but lacks antifungal activity against the yeasts P.pastoris GS115 and C.albicans.
 
References
"Isolation and characterization of a hepcidin peptide from the head kidney of large yellow croaker, Pseudosciaena crocea."
Zhang J., Yan Q., Ji R., Zou W., Guo G.
Fish Shellfish Immunol. 26:864-870(2009)
 
Function
Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages (By similarity).