PuroSPIN™ PCR Product Purification MIDIprep Kit
The PuroSPIN™ PCR Product Purification MIDIprep Kit is designed to provide a simple and easy-to-use method for purification of PCR DNA products (100 bp − 10 kb) from larger-volume enzymatic reactions, using MIDI-scale silica spin columns. It is also suitable for cleanup of linearized plasmid DNA after restriction enzyme digestion.
- The purification technology is based on nucleic acid binding to silica spin columns, which allows efficient removal of enzymes, protein, primers, nucleotides, salt and other contaminants. Silica spin columns offer a faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
- PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as sequencing, cloning and ligation, in vitro transcription, transfection, Southern blotting, qPCR, or microarray analysis.
MIDI spin columns
- Nucleic acid binding capacity of the PuroSPIN™ MIDI Spin Column: 1.2 mg of genomic DNA, 200 µg of plasmid DNA
- Load up to 4 mL of sample per spin
- Elute purified DNA in 1 mL of Elution Buffer EB or nuclease-free water
- Columns are supplied with 15 mL collection tubes; a standard benchtop centrifuge (5,000 x g) is sufficient
Available sizes
- 20 preps – NK103-20
- 50 preps – NK103-50
A 2-prep trial kit (NK103-2) is available on request. Available in Canada from Luna Nanotech, Markham, Ontario. Prices are in CAD; contact us for bulk or institutional quotes.
SKU List: NK103-20, NK103-50
Materials Safety Data Sheets (MSDS)
Product Specifications
Kit Type: PCR Product Purification Kit Format: Silica Spin Columns Kit Scale: MIDI PCR Product Size: 100 bp − 10 kb Column Binding Capacity: 1.2 mg of genomic DNA, 200 µg of plasmid DNA Sample Volume per Spin: Up to 4 mL Elution Volume: 1 mL Collection Tube: 15 mL Product Manual
$171.00Price
