Mycoplasma Elimination Reagent (SweMyco-1+2) (100×)

A two-component mycoplasma elimination treatment that is used in sequence: SweMyco-1 for four days, then SweMyco-2 for three days. Each component is supplied as 5 × 1 mL at 100X.
How it works
Mycoplasma have no cell wall, so antibiotics that act on cell walls do not work against them. This treatment uses quinolone, tetracycline and macrolide antibiotics, which attack mycoplasma through DNA replication and protein synthesis. Using the two components one after the other helps clear contamination while giving the cells a change of formulation partway through.
Key features
SweMyco-1 (5 × 1 mL) and SweMyco-2 (5 × 1 mL), 100X each
Quinolone, tetracycline and macrolide antibiotics
Used at 1:100: 4 days of component 1, then 3 days of component 2
How to use
Add SweMyco-1 to culture medium at 1:100 and culture for 4 days, changing medium as your protocol directs.
Switch to medium with SweMyco-2 at 1:100 and culture for 3 days.
Return to normal medium and re-test the culture for mycoplasma.
Specifications
Components: SweMyco-1: 5 × 1 mL; SweMyco-2: 5 × 1 mL
Strength: 100X each
Dilution: 1:100
Schedule: 4 days, then 3 days
Catalog number: LN-G4008-5ML