Polybrene, 10 mg/mL, Sterile-Filtered, Viral Transduction Enhancer, 1 mL
Polybrene (hexadimethrine bromide) supplied as a sterile-filtered 10 mg/mL solution, commonly added to improve viral transduction efficiency.
How it works
Viral particles and cell membranes both carry a negative charge, so they repel each other. Polybrene is a positively charged polymer that reduces this repulsion and helps lentiviral and retroviral particles reach and enter cells, which raises transduction efficiency.
Key features
- 10 mg/mL, 1 mL (10 mg)
- Sterile-filtered
- Hexadimethrine bromide
How to use
- Dilute the stock into culture medium to the working concentration for your cell type, since some cells are sensitive to it.
- Add the virus to the medium containing Polybrene and apply it to the cells.
- Change to fresh medium after the incubation time set by your protocol.
Specifications
- Compound: Polybrene (hexadimethrine bromide)
- Concentration: 10 mg/mL
- Size: 1 mL (10 mg)
- Sterility: Sterile-filtered
- Catalog number: LN-G1803-1ML

